Specially designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms.This kit combines DNA fragmentation, end repair and dA tailing into one step. The products can be directly used to adapter Ligation without purification, then for library enrichment and size selection. This kit is suitable for library preparation from 100 pg – 1 μg of input DNA. No mechanical fragmentation of the genome simplifies the process of library construction and shortens the operation time, suitable for the construction of PCR and PCR-Free libraries.This kit is perfectly compatible with DNA from different sources and different inputs, and the required fragment size library can be obtained by adjusting the fragmentation time according to the size of the target insert. All kit components are subjected to stringent functional quality control, ensuring the consistency and reproducibility of library preparation.