- Robust amplification without MgCl2 optimization
- High yields of PCR products
- High sensitivity and amplicon length
- Direct loading of PCR products on gels
DreamTaq DNA polymerase products
With green reaction buffer
How to video to demonstrate how to perform colony PCR as part of a cloning workflow using Thermo Scientific DreamTaq DNA Polymerase.
Ready-to-load PCR products
The DreamTaq Green format is a combination of DreamTaq DNA Polymerase and the Green Reaction Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on gels. The Green Buffer does not interfere with the performance of DreamTaq DNA Polymerase and is compatible with downstream applications including DNA sequencing, ligation and restriction digestion.
|Figure 1. Equal PCR performance with green and colorless reaction buffer. gDNA fragments were amplified with DreamTaq and DreamTaq Green DNA Polymerase with equal efficiency. DNA Ladder: Thermo Scientific™ GeneRuler™ Express DNA Ladder.||Figure 2. Reaction mixtures containing DreamTaq GreenReaction Buffer. (A) Before electrophoresis. (B) Blue and yellow tracking dyes after electrophoresis.|
Increased PCR sensitivity and amplicon yields
DreamTaq DNA Polymerase provides higher PCR sensitivity in comparison to conventional Taq enzymes. Robust amplification can be achieved even with very low amounts of template DNA.
Figure 3: Superior yields with low amounts of DNA template. A 545 bp fragment from the human α-L-Fucosidase gene was amplified with DreamTaq DNA Polymerase and Taq DNA Polymerases from other vendors according to manufacturers’ recommendations using decreasing amounts of template DNA.
- M – Thermo Scientific™ FastRuler™ Low Range DNA Ladder
- D – DreamTaq DNA Polymerase
- T – Thermo Scientific™ TaqDNA Polymerase
- 1-3 – TaqDNA polymerases from other vendors
Robust amplification of longer amplicons
DreamTaq DNA Polymerase outperforms conventional Taq enzymes, providing higher yields and amplifying longer amplicons. With DreamTaq DNA polymerase it is possible to amplify targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
Figure 4. Amplification of longer amplicons with DreamTaq DNA Polymerase. DNA fragments of increasing length were amplified with DreamTaq DNA Polymerase and Taq DNA polymerase from other vendors according to manufacturers’ recommendations. Only DreamTaq DNA Polymerase was able to amplify the longest (5000 bp) fragment. DNA Ladder: Thermo Scientific™ GeneRuler™ Express DNA Ladder.
|EP0702||DreamTaq DNA Polymerase||500 U|
|EP0703||DreamTaq DNA Polymerase||5 x 500 U|
|EP0712||DreamTaq Green DNA Polymerase||500 U|
|EP0713||DreamTaq Green DNA Polymerase||5×500 U|
|K1071||DreamTaq PCR Master Mix||200 rxns|
|K1072||DreamTaq PCR Master Mix||1,000 rxns|
|K1081||DreamTaq Green PCR Master Mix||200 rxns|
|K1082||DreamTaq Green PCR Master Mix||1,000 rxns|