InnuPure® C16 – Magnetic particle based extraction system
Exceptionally fast walk-away principle
The InnuPure® C16 is a flexible and efficient extraction system for fully automated isolation and purification of nucleic acids. The system, which was developed and manufactured in Germany, is designed for small and medium sample throughput and can process a wide range of starting materials. The system combines a unique liquid handling method with an extremely fast walk-away principle. Labor-intensive sample lysis steps are no longer necessary, as they are now incorporated into the automated extraction process in keeping with the starting material. The nucleic acids to be isolated are then adsorbed onto magnetic or paramagnetic particles whose surfaces have been specially adapted for this purpose. The required extraction chemistry has been optimized for the application at hand, allowing users to isolate high yields of very pure nucleic acids.
The fully automatic magnetic particle separation process is carried out in the wells of the plastic extraction vessels. After the starting material has been introduced into the isolation process, the necessary reagents are pipetted to the sample and then automatically removed by pipette tip.
Once the nucleic acids have been bound to magnetic particles, they collect at the bottom of the wells and, depending on the routine in use, are resolubilized by pipetting them in and out in an optimized process. Finally, the DNA and/or RNA are eluted into separate, capped Elution Tubes for direct storage or other applications. The extraction principle also efficiently prevents the cross contamination that often occurs in vacuum-based purification methods. In addition, the InnuPure® C16 is equipped with pre-installed application protocols in order to avoid time-consuming programming. The high flexibility of the system allows users to isolate DNA or RNA from up to 16 samples in parallel.
HID-Pro 320 for fast and easy operation
Users operate the InnuPure® C16 from a flexible, portable HID-Pro 320 unit with the large 5.7″ touchscreen. Because this PCbased system operates in a Windows CE environment, users have typical Windows functions and a clearly structured menu on the interface, making the entire system a stand-alone device. The real-time display allows scientists to check the status of the current extraction and follow each routine clearly and continuously. In addition, the USB interface makes it easy to update software and upload new isolation protocols.
Extremely easy handling thanks to optimized extraction kits
Extraction kits adapted to the InnuPure® C16 allow users to process forensic samples and isolate genomic DNA and viral or bacterial nucleic acids. These kits are ideal automation tools for efficiently isolating high-quality nucleic acids with no contamination. All purification kits are ready for use and have been optimized for different starting materials and quantities.
Fully pre-filled Reagent Strips and Plates minimize manual steps and save an impressive amount of time. In addition, the intelligent sample tray allows users to process individual samples. Therefore the positions of the deep-well Reagent Plates can be assembled with an additional adapter: a process that can be performed in just one easy step. This makes it possible to adapt up to 4 pre-filled Reagent Strips for individual sample handling and, as such, the InnuPure® C16 can be easily adapted to handle a quantity from 1 to 16 samples.
The system (including Sample Tray and extraction kits) is extremely fast and easy to use, allowing users to fully prep samples for nucleic acid isolation in just three steps.
Step 1: First, load the Sample Tray as shown with the appropriate accessories (You may wish to consult the kit user manual for details on how to position the Reagent Strips/Plates, elution vessels and Filter Tips.) The correct order for the prefilled, plastic reaction containers depends on the application. The number of elution vessels and Filter Tips depends on the number of samples to be processed.
Step 2: After loading the Sample Tray, position it in the InnuPure® C16 with the elution vessels in front. The Sample Tray will be drawn into the device automatically upon activation of the integrated soft-touch function.
Step 3: Finally, open the list with the pre-installed isolation protocols and the InnuPure® C16 will automatically run the extraction.
After the routine is finished, the Sample Tray containing the purified samples will automatically move out of the InnuPure® C16. The extraction process will take about 35 – 75 minutes depending on the application.
Instrument system, incl. user interface HID-Pro 320, Priming Station and Sample Tray for up to 2