Mutation Analysis with highest reproducibility
The new and unique Peltier element-based Temperature Gradient Gel Electrophoresis (TGGE) System from Biometra is a universal detection system designed especially for rapid mutation analysis.
By applying a temperature gradient during the electrophoretic separation of DNA or RNA, fragments of identical length but different sequence can be separated.
Mutation analysis without chemical gradient:
DNA fragments with point mutations will exhibit different melting behavior and, therefore, different conformation compared to the wild type DNA. By varying the denaturing conditions with a temperature gradient, TGGE achieves unparalleled separation and therefore sensitivity.
The TGGE System is superior to other gradient systems because of the linearity and reproducibility of its Peltier-controled temperature gradient. Chemical gradient such as those used in DGGE are not as reproducible, are difficult to establish and often do not completely resolve heteroduplices.
TGGE offers mutation analysis without ambiguity. Virtually 100% detection rate at less then a quarter of the time required by other systems!
