- Phusion® Hot Start II High-Fidelity DNA Polymerase
- The first choice for High-fidelity PCR
Finnzymes’ Phusion® DNA Polymerases offer PCR performance that no other enzyme can match.
- NEW: Phire® Hot Start II DNA Polymerase – Speed and specificity for routine and high-throughput PCR.
Finnzymes’ High Performance PCR offers high fidelity PCR performance with increased yields in just a fraction of the time when compared to conventional PCR.
We deconstructed the PCR process and created a tripartite solution that improves nearly every measurable aspect of PCR. It combines highly processive proofreading Phusion® DNA Polymerases, high-speed Piko® Thermal Cyclers, and ultra-thin walled UTW® tubes and plates. Each component alone is best in class. Together they offer synergies that propel PCR to unbeatable performance.
Speed – Significantly faster than any other combination
To save valuable research time, each component of the High Performance PCR solution is designed to expedite PCR protocols. Phusion DNA Polymerases are highly processive, due to a DNA-binding domain fused to the polymerase. The result is extreme reaction speed and robustness. The Phusion Flash PCR Master Mix requires only 15 seconds or less to extend a kilobase target. Clever designing enables the Piko Thermal Cycler to complete a 25-cycle PCR protocol in as little as 10 minutes. This speed improvement is based upon the fast ramp rate and an incredible settling time of less than 1 second, which allows for 0 second holds at annealing and denaturing steps. The vessels (UTW tubes and plates) have extremely thin walls where the tubes contact the cycler block. This results in less thermal resistance and rapid heat transfer from the block to the reaction mixture.
Yield – Higher efficiency amplification results in more product
High-speed PCR is often fickle – variable yields depend upon the purity of template and the need for tedious reaction optimization. This is not true for our integrated solution. Phusion technology offers several distinct advantages. First, we allow for high efficiency amplification under the most demanding conditions. Phusion DNA Polymerases have a very broad window for optimization that allows them to work robustly with common inhibitors of PCR (such as blood) and in varying reaction conditions. Second, the extreme stability of our Phusion DNA Polymerases at high temperatures, combined with the ultra-quick settling time of the Piko Thermal Cyclers, results in consistently abundant yields of specific product–even with prolonged PCR protocols.
Fidelity – Superior accuracy over Taq and Pfu based product
At the core of our unique polymerases is a cleverly engineered proofreading enzyme with enhanced DNA-binding activity and processivity. The Phusion technology improves the fidelity of the polymerase , such that Phusion High-Fidelity DNA Polymerases have the lowest error rates of any polymerases available. The error rate of Phusion DNA Polymerases is as low as 4.4 x 10-7 , which is more than 50-fold better than that of Taq DNA polymerase. High Performance PCR may be used in routine applications or with protocols requiring the highest accuracy.
Specificity – Reduced levels of primer-dimers and false-primed products
Primer-dimers and false-primed products are not only a nuisance, but may mask the correct target amplicon. Our integrated solution combats this by speeding up and heating up the reaction. The Piko Thermal Cycler uses fast ramping and short annealing times to reduce the effects of spurious amplification by minimizing false priming. These unwanted products are diminished by Phusion DNA Polymerases´ ability to dramatically raise annealing temperatures as compared with conventional PCR. To further improve specificity in PCR, Phusion DNA Polymerases are also available as hot start enzymes utilizing “zero-time reactivation.”