Phire® Animal Tissue Direct PCR Kit & Mastermix – DNA amplification directly from animal tissues.
Take the Direct route to PCR – Save time and money by amplifying DNA directly from sample material.
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, Dilution buffer and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species. A detailed manual is provided that describes protocols when working with different types of tissues.
• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).
The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
Two alternative protocols suitable for various purposes
In the direct protocol, a small piece of animal tissue (see recommended sample sizes here) is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1µl of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample, for example.
• 2 x 1.25 mL Phire Tissue Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 3 x 100 µL DNARelease Additive
• 40 µL Control Primer Mix (25 µM each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O’GeneRuler Express DNA Ladder (sufficient for 100 applications)
Storage and stability
Recommended storage temperature -20°C. Stable for one year from the packaging date
|Direct protocol||Dilution and storage protocol|
|Direct PCR Master Mixes|
|Phire Tissue Direct PCR Master Mix||100 x 50 µL rxns||250 x 20 µL rxns||F-170S|
|500 x 50 µL rxns||1,250 x 20 µL rxns||F-170L|
|Phire Plant Direct PCR Master Mix||100 x 50 µL rxns||250 x 20 µL rxns||F-160S|
|500 x 50 µL rxns||1,250 x 20 µL rxns||F-160L|
|Phusion Blood Direct PCR Master Mix||100 x 50 µL rxns||NA||F-175S|
|500 x 50 µL rxns||NA||F-175L|
|Direct PCR Kits|
|Phire Animal Tissue Direct PCR Kit||Kit 200 x 50 µL rxns||500 x 20 µL rxns||F-140WH|
|Phire Plant Direct PCR||Kit 200 x 50 µL rxns||500 x 20 µL rxns||F-130WH|
|Phusion Human Specimen Direct PCR Kit||Kit 200 x 50 µL rxns||500 x 20 µL rxns||F-150BID|
|Phusion Blood Direct PCR Kit||100 x 20 µL rxns||NA||F-547S|
|500 x 20 µL rxns||NA||F-547L|