Amresco Agaroses

Amresco – Choosing the Best-Suited Agarose

Agarose I™ (All-Purpose)
Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid and protein analytical and preparative applications. The high purity of Agarose I™ provides outstanding separation of biomolecules that retain full biological activity.

Agarose I™ has a low EEO for shorter electrophoretic runs without compromising resolution. Also, an excellent all-purpose agarose useful for blotting techniques and general manipulations.

Agarose I™, Biotechnology Grade (Top Quality), 250g (Tamar’s Cat. No. – AM-0710-250G)		Clalit Medical Engineering catalog number:			1-81008703-1
		Hebrew University catalog number:			102-15-300
		Rambam Medical Center catalog number:			212-0100-004
		Tel-Aviv University catalog number:			088915-3
		Volcani Center catalog number:			421-030-113
		Weizmann Institute catalog number:			2000-0002-052

Agarose RA™ (All-Purpose)
AMRESCO’s Agarose RA™ is a quality general application agarose that provides good resolution and is cost effective for high volume users. It was developed specifically for quick checks of PCR products, plasmids preps, screening and cloning. Agarose RA™ features high resolution, easy preparation and set up, a low EEO and excellent sensitivity with low background when stained.

 For special applications Amresco offers the following Agaroses:

Agarose SFR™ (Super Fine Resolution), developed at AMRESCO, is a high resolution sieving agarose with unsurpassed clarity. Agarose SFR rivals acrylamide in its ability to resolve DNA fragments less than 1,000 bp. DNA bands differing in size by 2% can be resolved in the range of 200 – 1,000 bp. This agarose is suitable for the analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats) and tetranucleotide repeats. The low melting temperature of Agarose SFR makes it an excellent medium for analytical and preparative electrophoresis.

Agarose 3:1 HRB™ (High Resolution Blend), developed at AMRESCO, is a unique mixture of agarose formulated to provide high resolution of small nucleic acids and PCR* products. Specially designed for analysis of DNA fragments less than 1,000 bp, Agarose 3:1 HRB displays precise banding patterns without smearing or high background fluorescence. The high gel strength allows for easy-to-handle gels that are optimal for blotting applications.

Agarose II™ (Low Melting), is a low-melting agarose that melts at temperatures between 62 – 68^OC and will remain for several hours as a liquid at 37^OC. These properties allow users to readily purify macromolecules from agarose slices or to conduct in-gel enzymatic manipulations. This superior quality agarose is ideal for digestions and ligations in the presence of remelted gel.

AGAROSE LF™ (Pulsed-Field Gel Electrophoresis), is optimized for intermediate and pulsed-field gel electrophoresis (PFGE) applications. The exceptionally low EEO and high gel strength of Agarose III facilitates faster electrophoresis running times in low concentration gels. This agarose is best suited for resolving large (>20 kb) DNA fragments.

Optimal Running Conditions
Product	Gel %		Optimal range for separation (bp)	Recommended buffer
Agarose I™ / RA™ (All- Purpose)	0. 8 1.00 1.20 2.00		800 – 22,000 500 – 10,000 400 – 7,000 250 – 5,000	TAE TAE/ TBE TAE/ TBE TBE
Agarose SFR™ (Super Fine Resolution) (compatible with in- gel manipulations)	2.00 3.00 4.00 5.00		500 – 2,000 100 – 1,200 50 – 500 20 – 250	TAE ‡ /TBE TAE ‡ /TBE TAE ‡ /TBE TAE ‡ /TBE
Agarose 3: 1 HRB™ (High Resolution Blend)	1.00 2.00 3.00 4.00		500 – 2,000 250 – 750 125 – 500 50 – 250	TBE TBE TBE TBE
Agarose II™ (Low Melting) (compatible with in-gel manipulations)	0.50 0.80 1.00 2.00		2,000 – 20,000 500 -15,000 200 – 10,000 100 – 1,000	TAE ‡ /TBE TAE ‡ /TBE TAE ‡ /TBE TAE ‡ /TBE
Agarose LF™ (Pulsed- Field Gel Electrophoresis)	0.50 0.70		10,000 – 40,000 1,000 – 20,000	TAE TAE
‡ Recommended for preparative electrophoresis, although TBE buffer will offer improved resolution of the smallest bands. TAE is more compatible with in- gel manipulations and band recovery procedures.