Specificity, Sensitivity, Reproducibility and convenience
Specificity – Maxima® Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Sensitivity – detects low copy number targets.
ROX Solution provided in a separate tube.
Wide linear range – accurate quantification across 9 orders of magnitude.
Universal – can be used on most real-time PCR instruments.
Reproducibility and convenience – ready-to-use 2X master mix.
Real-time PCR and RT-qPCR using SYBR® Green dye on most real-time PCR machines.
Maxima® SYBR Green qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes Maxima® Hot Start Taq DNA polymerase, SYBR® Green I dye and dNTPs in an optimized PCR buffer. ROX Solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima® Hot Start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima® SYBR Green qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Maxima® SYBR Green qPCR Master Mix (2X), ROX Solution provided
|Catalog#||Size, concentration||Supplied with:|
|Water, nuclease-free||ROX Solution (50 µM)|
|K0251||2 x 1.25 ml (for 250 react. of 20 µl)||2 x 1.25 ml||50.00 µl|
|K0252||10 x 1.25 ml (for 1250 react. of 20 µl)||10 x 1.25 ml||250.00 µl|
|K0253||4 x 12.5 ml (for 5000 react. of 20 µl)||2 x 30.00 ml||1.00 ml|