Finnzymes’ new DyNAmo™ ColorFlash qPCR Kits are a superior choice for fast, quantitative real-time analysis. These kits offer excellent performance in detection and quantification of DNA sequences from various sources. DyNAmo ColorFlash qPCR Kits are designed to ease qPCR setup and minimize errors by providing a simple way to track pipetting of reaction components.

The two kits, DyNAmo™ ColorFlash SYBR® Green qPCR Kit and DyNAmo™ ColorFlash Probe qPCR Kit, utilize an innovative color system: the 2x Master Mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, even when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Advantages

• Minimized risk of pipetting errors during reaction setup
• Also beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x Master Mix allows the use of UNG for prevention of carry-over contamination
• Licensed for qPCR

				Blue + yellow = green. Finnzymes’ DyNAmo ColorFlash qPCR Kits contain 2x Master Mix supplemented with a blue dye. The yellow 40x sample buffer is included as a separate vial. This buffer can be mixed with the samples to provide visual aid when pipetting the samples.
Master mix	Sample in buffer	qPCR reaction mix

 

Assists reaction setup with clear and white plates. White reaction vessels are especially good for qPCR applications as they deliver higher signal intensities than clear vessels. However, traditional colorless reaction components are poorly visible in white wells, making reaction setup more difficult. DyNAmo ColorFlash qPCR Kits overcome this difficulty and decrease the risk of pipetting errors. In the image, the blue wells contain only the 2x Master mix. The green color indicates that the sample DNA with yellow color has also been added into the reaction.

Components

DyNAmo™ ColorFlash SYBR® Green qPCR Kit	F-416S	F-416L
2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)	1 x 1ml (sufficient for 40 reactions of 50µl or 100 reactions of 20µl)	5  x 1ml (sufficient for 200 reactions of 50µl or 500 reactions of 20µl)
40x sample buffer solution with yellow dye	200µl	1000µl
50x ROX passive reference dye	1 x 50µl	1 x 250µl

 

DyNAmo™ ColorFlash Probe qPCR Kit	F-456S	F-456L
2x master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)	1 x 1ml (sufficient for 40 reactions of 50µl or 100 reactions of 20µl)	5  x 1ml (sufficient for 200 reactions of 50µl or 500 reactions of 20µl)
40x sample buffer solution with yellow dye	200µl	1000µl
50x ROX passive reference dye	1 x 50µl	1 x 250µl

Storage and stability

DyNAmo ColorFlash qPCR Kits are shipped on gel ice. Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit.

The yellow sample buffer solution is stable and can be stored at +4°C, but storage at -20°C with the other kit components is recommended.

The kits are stable for six months from the packaging date when stored and handled properly.

Ordering information

DyNAmo™ ColorFlash SYBR® Green qPCR Kit
F-416S	100 reactions (20µl each) or 40 reactions (50µl each)
F-416L	500 reactions (20µl each) or 200 reactions (50µl each)

 

DyNAmo™ ColorFlash Probe qPCR Kit
F-456S	100 reactions (20µl each) or 40 reactions (50µl each)
F-456L	500 reactions (20µl each) or 200 reactions (50µl each)