Finnzymes’ Phire® Hot Start II DNA Polymerase outperforms every Taq-based hot start polymerase on the market. This polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. Phire Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10-15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the unique hot start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments such as the Piko® Thermal Cycler from Finnzymes.

Innovative design for improved performance. Phire Hot Start II DNA Polymerase is constructed by fusing a novel DNA polymerase (orange) and a small dsDNA-binding protein (yellow). This technology dramatically increases the processivity of the polymerase thus improving its overall performance.

Advantages

• Quick hot start: No reactivation step
• Fast enzyme: Amplify four times faster than with hot start Taq
• Robust: Minimal reaction optimization due to high inhibitor tolerance
• High yields: Abundant products due to high efficiency
• Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq

Quick hot start

Phire Hot Start II DNA Polymerase utilizes Affibody® inactivation technology, which reversibly inactivates the DNA polymerase at room temperature. At polymerization temperature the Affibody ligand is released from the DNA polymerase. This completely eliminates the need for a DNA polymerase reactivation step in cycling protocol, thus speeding up PCR even more.

Fast PCR with high yields

The DNA-binding domain of Phire Hot Start II DNA Polymerase stabilizes the polymerase-DNA complex during extension steps in PCR. This enables the polymerase to amplify DNA four times faster than standard Taq DNA polymerase. The high efficiency also results in abundant yields.

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Comparison of PCR cycling times	Abundant yields in shorter time

Longer PCR products

Phire Hot Start II DNA Polymerase is capable of amplifying longer DNA fragments than any hot start Taq DNA polymerase on the market.

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Amplification of long fragments

Contents

Phire Hot Start II DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer contains 7.5 mM MgCl₂ in the provided 5x concentration.

Storage and stability

Recommended storage temperature -20°C. Stable for one year from the assay date

Ordering information

Phire® Hot Start II DNA Polymerase
F-122S	200 reactions of 50 µl (200 µl)
F-122L	1000 reactions of 50 µl (1.0 ml)