Take the Direct route to PCR - Save time and money by amplifying DNA directly from sample material.

Finnzymes’ Phire® Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. No DNA isolation is required prior to PCR. The kit is based on Phire® Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in unpurified animal tissues. This kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools and DNARelease™ Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers that can be used with numerous animal species. When combined with Finnzymes’ Piko® Thermal Cycler and UTW® vessels, PCR protocols can be completed in as little as 30 minutes.

Advantages

• No need for time-consuming DNA extraction
• Phire Hot Start II DNA Polymerase delivers high inhibitor tolerance, specificity and high yields
• Fast and simple protocols require minimal hands-on time
• Very little sample material required
• Compatible with tissues from various animal species, including mice, fish and insects (see complete list of tested animal tissues here)

Two alternative protocols suitable for various purposes

In the direct protocol, a small piece of animal tissue (see recommended sample sizes here) is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1µl  of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample, for example.

Fast and reliable PCR from various tissue samples from a number of species

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Direct PCR from various animal tissues	Genotyping F2 transgenic mice

Kit components

Kit component F-140 Phire® Hot Start II DNA Polymerase 200 µl 2x Phire® Animal Tissue PCR Buffer (includes dNTPs and MgCl2) 5 x 1 ml Universal control primer mix 40 µl Dilution Buffer 5 ml DNARelease™ Additive 3 x 100 µl Gel loading dye 3 x 1 ml Harris Uni-Core™ 0.50 mm 1 Harris Cutting Mat™ 1
	Control primer sequences for zebrafish and Drosophila

Storage and stability

Recommended storage temperature -20°C. Stable for one year from the packaging date

Ordering information

Phire® Animal Tissue Direct PCR Kit
F-140	200 reactions (50µl each)