The flexible Eco system is an open platform that supports all chemistries and Real-Time PCR applications, including absolute quantification by standard curve, relative quantification using the delta delta Cq method with support for multiple reference gene normalization, allelic discrimination by end-point fluorescence, and gene scanning and genotyping by High Resolution Melt (HRM) curve analysis.
| Instrument |
|
|
Thermal system |
Single Peltier-based system |
|
Block format |
48-well block |
|
Consumables |
48-well Eco plates and optical Eco adhesive seals |
|
Accessories |
Eco sample loading dock |
|
Sample volumes |
5–20µl validated (optimized for standard 20µl protocols) |
|
Average block ramp rate |
5.5°C/sec |
|
Temperature range |
35–100°C |
|
Temperature uniformity |
± 0.1°C |
|
Optical system |
LED excitation (452–486 nm and 542–582 nm), four emission filters (505–545 nm, 562–596 nm, 604–644 nm, and 665–705 nm) and CCD camera |
|
Calibrated dyes at shipment |
SYBR, FAM, HEX, ROX, Cy5: factory-calibrated. Additional dyes within wavelength range of Eco filters are supported with no additional calibration required for implementation |
|
Data collection |
Data collected in all four filters for all wells regardless of plate setup; plate setup for data analysis can be altered after run completion.
Melt curve analysis supports continuous data acquisition in a single channel, providing increased data point collection and reduced run times |
|
Real-Time PCR run time (40 cycles) |
Less than 40 minutes |
|
Electrical |
Voltage: 120VAC=10%
Nominal current draw: 8A
Frequency: 50/60 Hz=1%
Peak Power 500VA, typical power is 180VA |
|
Software |
Eco System Software supports all chemistries and a variety of applications, including absolute quantification, relative quantification, allelic discrimination, and high resolution melt curve analysis (HRM) |
|
Warranty |
12-month warranty (includes parts and labor) |
Instrument Physical
|
|
|
Instrument dimensions |
12.6" closed (height)
14.5" open (height)
13.6" (depth)
12.2" (width)
32 cm closed (height)
36.8 cm open (height)
34.5 cm (depth)
31 cm (width) |
|
Weight |
30 lbs (13.6 Kg) |
| Performance |
|
|
Sensitivity |
1 copy |
|
Dynamic range |
9 logs linear range |
|
Precision |
Discriminates 5,000 and 10,000 template copies with 99% confidence |
