Specially designed for the construction of small RNA library for next generation sequencing (NGS) on Illumina platforms. The input template for this kit can be 0.1 μg-1 μg of animal or plant total RNA or purified small RNA. Small RNA libraries applicable for Illumina NGS platforms are prepared by the ligation of universal adapter on the 3’-end and 5’-end of the small RNA, respectively, followed by reverse transcription, PCR amplification, and purification by PAGE gel or magnetic beads. This kit contains all the necessary enzymes and buffers for library preparation and purification. All kit components are subjected to stringent functional quality control, ensuring the consistency and reproducibility of library preparation.