Cleavage Under Targets and Tagmentation (CUT&Tag) is a specific technology designed for the study of protein-genomic interaction that can seamlessly replace the traditional ChIP-Seq. Compared with ChIP-Seq, the CUT&Tag has several significant advantages, including high signal-to-noise ratio, excellent repeatability, short operation time (generate sequencing-ready libraries beginning with live cells within one day), and low cell input. This technology will facilitates the research in epigenetics, tumors, and stem cells.
Hyperactive In-Situ ChIP Library Prep Kit for Illumina® (#TD901/2)
Hyperactive In-Situ ChIP Library Prep Kit for Illumina is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology. CUT&Tag technology is a new methodfor research on protein-genomic interaction by fusion of Protein G or Protein A with engineered ultra-active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG-Tn5 / pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing.
Hyperactive pG/pA-Tn5 Transposon for Illumina®(4 μM) (#S612 /3)
Hyperactive pG/pA-Tn5 Transposon is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology which is a new
method for research on protein-genomic interaction.
Hyperactive pG/pA -Tn5 Transposon is pre-loaded with Adapter, also is the fusion of Protein G with engineered ultra – active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG/pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing.
Compared with the traditional protein-genomic interaction research method of ChIP-Seq, CUT&Tag has significant advantages of low cell input, short operation time, high signal-to-noise ratio, good repeatability and is especially suitable for research on early embryo development, stem cells, tumors, and epigenetics.