This kit of Vazyme, uses chemiluminescence method to detect protein or nucleic acid biological macromolecules. The principle is that the protein or nucleic acid is transferred to the blotting membrane after electrophoresis, and the primary antibody and horseradish peroxidase HRP-labeled secondary antibody bind to the target protein on the membrane, or the HRP-labeled probe directly or indirectly binds to the membrane. On the nucleic acid. After washing the membrane, incubate the membrane with the ECL working solution prepared by this product at room temperature under visible light for a few minutes, and coat the blot membrane with plastic wrap and fix it on the X-ray exposure cassette. Then go to a dark room and press the X-ray film on the film to expose for a few seconds to a few minutes to a few hours. After development and fixation, protein or nucleic acid bands can be clearly displayed on X-ray film. It is also possible to scan the blot film directly without X-ray film exposure.
High-quality western developing reagents; optimized formula to enhance luminous intensity; strong anti-quenching ability; compatible with film exposure and CCD scanning
√ High-quality western developing reagent
√ Optimized formula to enhance luminous intensity
√ Strong anti-quenching ability
√ Compatible with film exposure and CCD scanning
Vazyme High-Sensitivity ECL Chemiluminescence Detection Kit (Ready-to-Use):