5min TA/Blunt-Zero Cloning Kit
- Ready-to-use master mix.
- Suitable for both TA cloning and blunt-end cloning.
- Rapid cloning within 5 min.
- High cloning efficiency with Positive Clone Rate > 95%.
- Ampicillin and Kana dual resistance vector
5min TM TA/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vectorcontaining the suicide gene ccdB and a blunt end factor. Combining with the optimal buffer, the second generation of Topoisomeraseprovides a highly efficient, 5 minute, one-step cloning strategy at room temperature. This product using a vector containing the suicidegene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grownormally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5min TMTA/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.