Isothermal AmpliFire and Pathogen Detection Kits.
AmplifyRP XRT and XRT kits provide real-time isothermal nucleic acid amplification and detection in a portable and easy-to-use format. Using AmplifyRP, end-users can obtain the same high level of sensitivity and specificity as PCR but with some distinct advantages.
Recombinase-Polymerase Isothermal Amplification Pathogen specific kits:
- Crude sample preparation
- Ready-to-use amplification reagents
- Pre-optimized and validated
- Real-time results in 30 minutes or less
- Lower equipment cost than PCR / qPCR
AmplifyRP® Discovery Kits and Master Mixes for LAMP and RPA
AmplifyRP Discovery Kits provide all the reagents necessary to amplify DNA at a single operating temperature (39°C) lyophilized into individual reaction pellets. The end-user can design their own primers and probes for a specific target, mix them with our amplification reagents, and get results (generally in less than 30 minutes). RNA amplification is also possible with the addition of reverse transcriptase.
Simple testing process:
- Make a Master Mix using a supplied rehydration buffer and the primers & probe that you design
- Rehydrate the reaction pellets included in the kit using the Master Mix
- Heat the reaction at 39°C for 15 to 30 minutes. This step may be done using a portable heat block for end-point detection (Acceler8) OR using a portable fluorometer which will also display results in real-time (XRT)
- Get results!
Features
- Crude sample extraction. No DNA or RNA purification required
- Amplify trace levels of template in minutes
- Deployable in the laboratory or the field
- Quick results. Total assay time less than 30 minutes including sample prep
- Primer / probe design similar to PCR methods and is backed by a dedicated technical support group
- Collaborative and / or contract assay design opportunities