T7-FlashScribe™ Transcription Kit (Manual)
For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1µg DNA in 30 minutes.
| C-ASF3507 | 50 reactions |
mRNA Decapping
Cap-Clip™ Acid Pyrophosphatase (Manual)
For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5′-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.
| C-CC15011H | 100 units |
Modified NTP In Vitro Transcription
INCOGNITO™ T7 Ψ-RNA Transcription Kit (Manual)
For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1µg DNA in 2 hours.
| C-ICTY110510 | 10 reactions |
INCOGNITO™ SP6 Ψ-RNA Transcription Kit (Manual)
For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1µg DNA in 2 hours.
| C-ICSY110510 | 10 reactions |
INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit (Manual)
For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells.
A reaction produces 100 µg RNA from 1µg DNA in 2 hours.
| C-ICTMY110510 | 10 reactions |
INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit (Manual)
For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA).
5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40µg RNA from 1µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.
| C-ICTAMY110510 | 10 reactions |