GeneAll® Ribospin™ Plant
GeneAll® Ribospin™ Plant is specially designed for purification of total RNA from various plant tissues such as leaves, stems, roots and picky plant samples. This kit provides the optimized buffer and spin column, which is effective in removing polysaccharides and polyphenolic compounds and isolating intact plant RNA. The procedure of Ribospin™ Plant begins with the disruption of sample in liquid nitrogen using mortar and pestle. The disrupted sample can be lysed in Buffer RPL or REL. Most impurities except RNA in the lysate are eliminated by filtration through EzPure™ Filter and total RNA including a little impurity is bound to the membrane of Column Type W. Survived genomic DNA can be exterminated by on-column DNase I treatment. Whole procedure of Ribospin™ Plant takes only 25 minutes.
GeneAll® Ribospin™ Seed / Fruit kit
GeneAll® Ribospin™ Seed / Fruit is designed for easy and convenient isolation of total RNA from difficult plant tissues such as seeds, fruits, and rhizomes. Especially, this kit can remove effectively large quantities of secondary metabolites including polysaccharides and polyphenolic compounds which can lead to inhibition of downstream application. For efficient RNA purification, this kit offers optimized lysis system according to the sample type and adopts EzPure™ Filter to eliminate impurities simply from lysate. Moreover, contamination of genomic DNA, that causes interference in RNA analysis, can be excluded by on-column DNase I treatment in these procedures.