Nordmark offers most commercially available collagenases are derived by fermentation of the natural collagenase-producing bacteria species Clostridium histolyticum. The strain of C. histolyticum used in the manufacturing of our collagenase products has been carefully selected for the production of large amounts of collagenases and balanced amounts of other proteolytic activities like neutral protease and clostripain.
As a pathogen, C. histolyticum uses these enzymatic activities to infect a potential host by digesting the extracellular matrix proteins of connective tissues. Therefore, the natural composition of these enzymes (collagenase class I and class II, neutral protease and clostripain) is already optimized by nature for the most efficient tissue dissociation resulting in a high yield of viable cells.
Every application of collagenases places different demands on optimal digestion conditions. Nordmark Collagenase NB products range from standard collagenases containing balanced amounts of other proteolytic activities to highly purified collagenases, in research or GMP grades, respectively. For this reason, our collagenase products are exceptionally suitable for a variety of cell isolation applications.