Supporting Films and Fabrics for Casting Gels
GEL-FIX™ products are sturdy supports for gels to be incorporated during casting. GEL FIX™ is made from polyester film. Both surfaces of the film are chemically activated providing covalent binding to the gel layer. The gel will adhere to the GEL-FIX™ film not only during electrophoresis but in all subsequent staining and fixing procedures. Thus, it supports the gel layer maintaining exact shape and size (no shrinking, no swelling, no tearing). For documentation, the film-bound gel can be air-dried just as easy at room temperature to result in a transparent film, ideally suited to store the original gel in the laboratory notebook or to acquire it for electronic date processing. GEL-FIX™ for Covers is used to protect the surface of a cast gel from drying out but prevents the gel from adhesion. NetFix™ is an inert, reinforcing fabric which serves as an ideal support for gel layers. If the polyacrylamide gel is subject to blotting after electrophoresis we recommend NetFix™ for PAG. The polyester fabric is activated to bind polyacrylamide.
- GEL-FIX™ can be cut into every desired size
- GEL-FIX™ is stable to temperatures up to 110 °C
- GEL-FIX™ shelf life time is 24 months if stored dry/dark
- GEL-FIX™ is transparent to UV light above 310 nm
- NetFix™ for blotting
Reagents and Accessories to Cast and Run Gels
SERVA‘s ready-to-use acrylamide/bis, acrylamide and N,N’-methylene bisacrylamide stock solutions allow you to cast your own gels with the desired acrylamide/ bisacrylamide ratio. The solutions are made from highly purified acrylamide and N,N’- methylene bisacrylamide, stabilized and application tested in isoelectric focusing. Minimize your health risk and optimize your results. Agarose SERVA neutral for IEF is a premium grade agarose for isoelectric focusing, chemically treated to neutralize residual negative charge sites, virtually eliminating electroendosmosis. Reagents like buffers and cooling contact fluid are available as well as accessories like applicator strips, cooling contact fluid, electrode wicks or glass plates.
SERVA Stains for IEF Gels
Depending on the required sensitivity and/or application IEF gels may be stained either by SERVA Blue W, SERVA Violet 17 or by silver. The SERVA Violet 17 Staining Kit is a fast and convenient solution for rapid staining in routine applications. SERVA offers two different silver staining kits for highly sensitive staining of protein bands. For staining gels in diagnostics of Multiple Sclerosis (MS) the SERVA CSF Silver Staining Kit has been developed. Besides kits as complete solutions a comprehensive product line of dyes and reagents for staining IEF gels is available.