UltraScript™ cDNA Synthesis Kit
When synthesizing a protein, DNA is transcribed into mRNA which is then translated into a protein. One difference between eukaryotic and prokaryotic genes is that eukaryotic genes often contain introns which are not coding sequences, in contrast with exons, which are DNA coding sequences. During transcription, intronic RNA is excised from the RNA primary transcript and the remaining pieces of the RNA primary transcript are spliced back together resulting in processed mRNA. The mRNA code is then translated into an amino acid chain that comprises the newly made protein.
Using reverse transcriptase polymerases, DNA can be synthesised from mRNA and total RNA. Thus it is a ‘complementary’ copy of the mRNA, and is thus called complementary DNA (cDNA). cDNA forms the substrate for the majority of qPCR gene expression experiments.
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Ordering Info
A&A TranScriba noGenome Kit
The TranScriba noGenome™ kit utilises minimum hands-on time and effort compared to already existing DNA removal protocols combined with reverse transcription. It consists of two consecutive steps: efficiently getting rid of genomic DNA and first-strand cDNA, high yield synthesis.
The RNA sample is briefly incubated in the noGenome™ buffer to effectively remove genomic DNA. After genomic DNA elimination, the pure RNA sample is ready for reverse transcription. The reverse transcription may take from 15 to 60 min depending on target RNA features and template quality.
TranScriba™ reverse transcriptase has a high affinity for RNA and is optimized for efficient and sensitive cDNA synthesis from 10 pg to 1 μg of RNA. This high affinity, in combination with TranScriba™ thermostability and reaction buffer, enables high cDNA yields, even from templates with high GC-content or complex secondary structures.
Ordering Info
| Cat Number | Product Name | Size |
|---|---|---|
| 4000NG-20 | TranScriba noGenome, first-strand cDNA synthesis with efficient genomic DNA removal | 20×20µl reactions |
| 4000NG-100 | TranScriba noGenome, first-strand cDNA synthesis with efficient genomic DNA removal | 100×20µl reactions |