Tornado™ DNAse
(#1009-10T, 1009-50T)
Tornado™ DNAse (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complexes. The enzyme contains an additional domain responsible for the effective binding of a substrate DNA. The result is a versatile nuclease that possesses remarkably higher affinity towards dsDNA, even in the nM range of the DNA concentration.
Applications
- removal of contaminating genomic DNA from RNA samples
- removal DNA matrix post in vitro transcription.
- study of DNA-protein interactions by DNase I footprinting
- preventing false positive PCR results in one-step RT-qPCR
DNAse I
(#1009-10, 1009-100)
DNase (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complex. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ and Mn²⁺ ions.
Enzyme is purified from P.pastoris expressing bovine pancreas DNAse I gene. DNAse I may be used to degrade DNA in applications that are sensitive to the presence of RNAses.
Application
● removal of contaminating genomic DNA from RNA samples.
● DNA labeling by nick-translation.
● studies of DNA-protein interactions by DNase I footprinting.
RNAse A
Nuclease for RNA degradation. DNAse free.
(#1006-10, 1006-50)
RNase A is an endoribonuclease that specifically degrades ssRNA at C and U residues.
The enzyme is active depending on the reaction conditions. At low salt concentration (<100 mM NaCl) RNAse cleaves ssRNA, dsRNA and RNA in DNA-RNA complex. At high salt concentration (>300 mM NaCl) RNAse specifically cleaves ssRNA only.
Enzyme is purified from bovine pancreas extracts. RNAse A may be used to degrade RNA in applications that are sensitive to the presence of DNAses.
Application
● removal of RNA from recombinant protein preparation.
● mapping single-base mutation in DNA or RNA.
● plasmid and genomic DNA preparation.
RNAse R
(#RRT250325)
- Ideal for isolating circular RNA (circRNA) from total RNA preparations.
- Rapid workflow providing digestion of 5 µg of linear RNA with 1 µg of RNase R in just 30 minutes.
- Preserves circRNA, lariat intron RNA and dsRNA while digesting linear RNA and Y-structure RNA.
Ribonuclease R (RNase R) from E. coli is a 3’→5’ exoribonuclease that digests linear RNA and Y-structure RNA but does not digest circular RNA (circRNA), lariat intron RNA, blunt-ended double-stranded RNA (dsRNA), dsRNA with 3’-overhangs <7 nt in length or single-stranded DNA. Highly structured linear cellular RNAs such as prokaryotic 23S and 16S ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs), transfer RNAs (tRNAs) and histone mRNAs are also not efficiently degraded by RNase R. 1 µg of RNase R will digest 5 µg of linear RNA in just 30 minutes at 37°C.
RNase R is ideal for circRNA isolation from total cellular RNA preparations for use in intron and splicing studies as well as for natural cellular circRNA identification. Additionally, RNase R is a crucial component in in vitro circRNA preparation cleanup and enrichment with both RNA Ligase-based or self-splicing permutated intron–exon sequence (PIE method)-based methodologies.
Exonuclease I
(#1020-1, 1020-5)
Recombinant Exonuclease I removal of nucleotides from ssDNA in the 3’-5’ direction.
Application
● removal of ssDNA with a hydroxyl group at the 3-end
● removing primer residues in the mixture after DNA amplification
● when used simultaneously with alkaline phosphatase, removes primers and nucleotides
Also available: Exonuclease V
Viscolase™
Highly efficient nuclease degrading DNA and RNA. Reduces viscosity of the protein preparations.
(#1010-25, 1010-100)
Viscolase™ is a recombinant nuclease with a broad spectrum of nucleolytic activity. It is encoded by the same gene as Benzonase®. Viscolase™ is produced in a unique yeast protein expression system. This versatile endonuclease attacks and degrades all forms of DNA and RNA (both single and double stranded as well as linear and circular forms).
Viscolase™ maintains its extremely high nucleolytic activity over a wide range of operating conditions.
Applications
• Viscosity reduction in cellular lysates and protein extracts
• Sample preparation for 2D gel electrophoresis
• Removal of nucleic acid contaminants from recombinant protein preparations
Advantages
• Viscolase™ is provided in very high purity (over 99%).
• The purification procedure protects the enzyme against impurities such as other proteins including proteases.
• Dilluted enzyme retains the appropriate activity of the enzyme.