Amazing protein yields in suspension CHO & HEK-293 cells at an affordable price
High Protein Yield – FectoPRO™ by Polyplus Transfections
- Amazing protein and antibody production yields
- Cost-effective Transient Gene Expression: Uses lower plasmid DNA amount
- Sustained protein production over several days
- Easily scalable
- Reproducible production yields
- Compatible with various mammalian expression media & cells systems
- Compliant with Biomanufacturing guidelines
For the first time, achieve amazing protein yields using transient transfection and gene expression.
FectoPRO™ Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture). FectoPRO™-mediated transfection process is easily scalable from a few ml to several liters of cell culture, ensuring robust reproducible protein production. Increased productivity and low DNA amount requirement make FectoPRO™ a cost-effective solution for bioproduction processes.
FectoPRO™ largely outperforms other transfection reagents commonly used in Bioproduction processes in terms of transfection efficiency (Fig. 2), leading to superior protein production yields (Fig. 3).
Fig. 1. FectoPRO™ gives high transfection efficiency in suspension CHO cells. FreeStyle™ CHO-S cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyle™ CHO Expression Medium and transfected with FectoPRO™ + FectoPRO™ Booster or FreeStyle™ MAX Reagent using 0.4 µg/ml and 1.25 µg/ml of a GFP expressing plasmid, respectively. GFP expression was assayed 24 h after transfection using fluorescence microscopy.
Fig. 2. FectoPRO™ gives superior protein production yields in suspension CHO and HEK-293 cells. FreeStyle™ CHO-S and HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyle™ CHO or 293 Expression Medium and transfected with FectoPRO™ + FectoPRO™ Booster (0.4 µg DNA/ml), FreeStyle™ MAX Reagent (1.25 µg DNA/ml) or PEIpro™ (1 µg DNA/ml) following recommended protocols for the respective reagents. IgG3-Fc (34 kDa) production was assayed 72 h (CHO cells) and 120 h (HEK-293 cells) after transfection by protein G affinity quantification (HPLC) and Western Blot. Standard corresponds to purified IgG3-Fc (34 kDa). (FreeStyle™ & 293fectin™ are trademarks of Life Technologies™ Corporation.)
Ordering information :
|Catalog Number||Volume of FectoPRO™Reagent||Volume of FectoPRO™ Booster|
|116-001||1 ml||1 ml|
|116-010||10 ml||10 ml|
|116-100||10 x 10 ml||10 x 10 ml|
|Catalog number||Volume of reagent|
|FectoPRO™ Booster||704-10||10 ml|