Recombinant Monoclonal Antibodies
Adipogen Recombinant Monoclonal Antibodies – developed from a NON-ANIMAL SOURCE using in vitro antibody phage display technology.
- Developed from a human antibody phage display library.
- Consists of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) fused to a Fc region.
- Produced in mammalian cells (CHO or HEK 293).
- Similar properties compared to monoclonal antibodies developed in mice / rat (e.g. affinity in the low nanomolar range).
- Standard secondary antibodies can be used.
- Ideal for conserved antigens (which are poorly immunogenic in animals).
- Detect conformational epitopes (e.g. GTP-bound proteins).
- Detect protein modifications (e.g. phosphorylations, ubiquitinations).
- Possibility to exchange the Fc region with Fc from other species.